Product Description
TransPLEX® C-WTA Kit is optimized for the amplification of RNA from difficult samples such as FFPE and serum, providing uniform representation across the entire transcript. The reagent formulations have been designed and optimized to yield a large library from as little as 10 ng of input RNA from fresh tissues and cells or 25 ng from FFPE. The complete TransPLEX C-WTA workflow is performed in a single tube, reducing workflow and minimizing risk of contamination or sample mis-identification.
C-WTA Kit is available for diagnostic use only; cGMP certification ensures ready use for clinical assays
Advantages
- Proven technology with simple workflow that can be automated
- Generates isothermal library without 3′-bias followed by amplification
- Amplifies 10 ng – 300 ng of RNA or formalin-fixed RNA for microarray, PCR, or sequencing assays
- High success rate (>90%) with FFPE Specimens
- Manufactured under cGMPs
Specification
Store at –20 °C.
Guaranteed for 12 months at -20°C in a constant temperature freezer.
Kit selection guide
| Platform | Applications | Sample | Input Amount | Technology | |
| ThruPLEX® DNA-seq Kit | Illumina NGS | WGS, WES, Targeted Sequencing, ChIP-seq, RNA-seq | FFPE DNA, gDNA, cDNA, Cell-free DNA, ChIP DNA, amplicons | 50 pg – 50 ng | High sensitivity ligation-based amplification |
| ThruPLEX® Plasma-seq Kit* | Illumina NGS | WGS, WES, Targeted Sequencing, ctDNA Analysis | Cell-free DNA isolated from plasma | <1 ng – 30 ng | High sensitivity ligation-based amplification |
| PicoPLEX® DNA-seq Kit | Illumina NGS | CNV, Aneuploidy | Eukaryotic Cell(s) or gDNA | <6 pg – 60pg | Quasi-random primed linear amplification |
| PicoPLEX® WGA Kit | Arrays, PCR | CNV, Aneuploidy | Eukaryotic Cell(s) or gDNA | <6 pg – 60 pg DNA | Quasi-random primed linear amplification |
| TransPLEX® C-WTA Kit | Arrays, PCR | Gene Expression Analysis | RNA | 10 – 300 ng RNA | Quasi-random primed amplification |
