Next Generation Sequencing Services

Genome Sequencing

The general whole-genome sequencing workflow starts with the generation of sequencer-ready DNA fragment libraries from genomic DNA. For whole-genome sequencing, the combination of short inserts and longer reads allow characterization of any genome. For de novo WGS, the unparalleled raw read accuracy of Illumina next-generation sequencing (NGS) technology provides high quality, long contig assemblies.

Exome Sequencing

The exome represents less than 2% of the human genome, but contains ~85% of known disease-causing variants, making whole-exome sequencing a cost-effective alternative to whole-genome sequencing. Using exome sequencing data you can investigate the protein coding regions of the genome when sequencing an entire genome is not practical or necessary. It can efficiently identify variants across a wide range of applications, including population genetics, genetic disease, and cancer studies. InterpretOmics offers comprehensive exome sequencing solutions, from library preparation to data analysis.

16s rRNA Sequencing

16S ribosomal RNA (rRNA) sequencing is a common amplicon sequencing method used to identify and compare bacteria present within a given sample. 16S rRNA gene sequencing is a well-established method for studying phylogeny and taxonomy of samples from complex microbiomes or environments that are difficult or impossible to study such as Fecal, Soil, gut etc…,

Transcriptome Sequencing

With no probes or primers to design, mRNA sequencing (mRNA-Seq) delivers unbiased and unparalleled information about the transcriptome. InterpretOmics delivers high quality data even from a minimum of 250 ng of Total RNA as well FFPE derived RNA.

Small RNA Sequencing

Small RNA analysis is a powerful application, enabling the discovery and profiling of microRNAs and other non-coding RNA for any organism, without prior genome annotation. Using low RNA inputs (1 uG) , you can profile the differential expression of known microRNAs and other small non-coding RNAs, as well as detect novel microRNA targets and wide-ranging sequence variation or “iso-miR” miRBase accessions.

ChIP Sequencing

ChIP seq is a method used to analyze DNA-Protein interactions. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global binding sites precisely for any protein of interest. We will deliver high quality data even from 15 ngs of ChIP DNA.

Methylation Sequencing

Sequencing-based DNA methylation analysis applies the coverage density and flexibility enabled by next-generation sequencing to enhance epigenetic studies. We require high quality with the total amount of 1000ng in order to proceed with bisulfite conversion followed by library preparation and sequencing.

Data Analysis and Interpretation Services

– De novo as well as reference based genome annotation, phylogeny, structural and functional analysis.

– Transcriptome mapping for differentially regulated genes between cohorts and meta-analysis for disease mapping, small RNA driven regulation of genes

– Epigenetic analyses for genetic regulation.

– WGS and WES based discovery services for variation analyses and interpretation.