Easy® EGFR

Product Code:

The Easy®EGFR kits allow the qualitative detection of somatic mutations in EGFR gene by real-time PCR combined with a mutant enrichment technique.

Product Description

Detection of the main mutations of exons 18, 19, 20, 21 of EGFR gene using 8 oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene. A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA in each sample


Control DNA positive for all the mutations detected by the kit. Reference standard DNA Horizon EGFR ΔE746-A750 1% to monitor the analytical process and the performances of the system. The standard is characterized by a well defined allelic ratio wild-type/mutant.


The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAM and HEX (LOD down to 0.5%).

Starting material

he kit allows the analysis of DNA extracted from fresh, frozen and formalin-fixed paraffin-embedded tissues and of circulating tumor DNA extracted from plasma.

Execution time

2 hours


Easy® KRAS Manual