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Poly(A) RNA Selection Kit

Company:
  • Highly specific poly(A) enrichment
  • Magnetic bead-based purification
  • Various downstream applications such as RNA-Seq
  • Fast workflow

Product Description

The Poly(A) RNA Selection Module enables the rapid and highly specific enrichment of polyadenylated RNAs from total RNA samples.

Performance

Rapid Turnaround

Polyadenylated RNAs can be isolated from total RNA samples within about one hour.

Various Downstream Applications

Isolated mRNA can directly be used for RNA-Seq library preparation (e.g., SENSE Total RNA-Seq kit), SAGE, CAGE, cloning, microarrays, cDNA synthesis, and others

Highly Specific for Poly(A) RNA

Other RNA species (rRNA and tRNA) do not contain poly(A) sequences and therefore will not bind to the oligo(dT) beads.
PolyA_Graph1
Figure 1. Bioanalyzer traces of the poly(A) RNA enrichment using 5 µg of Universal Human Reference RNA (UHRR) (A) input with the Poly(A) RNA Selection Kit. Recovery of poly(A) selected UHRR (B) is about 2 % of the initial input amo.

Workflow

Step 1 : Preparation of Beads and Denaturation of RNAStep

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The total RNA Is briefly denatured and magnetic beads are aliquoted and washed.

Step 2 : Hybridization of poly(A) RNA

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The polyadenylated 3’ ends present in most mRNAs are hybridized to oligodT beads.

Step 3 : Poly(A) RNA Enrichment

03polya_workflow1
Any RNA without poly(A) stretches, such as rRNA and tRNA,will not be captured by the oligodT beads and will be washed away.

Step 4 : Poly(A) RNA Elution

04polya_workflow
The poly(A) RNA is eluted from the olidodT beads by heating in water.

FAQs

1. What is the length of the oligodT?
2. Which RNAs get isolated using the Poly(A) RNA Selection Module?
3. Can I use the Poly(A) RNA Selection Kit for the SENSE library generation?
4. Do I need two rounds of RNA-binding?
5. What is the poly(A) RNA recovery?

Ordering Information

Kit Reactions Cat.No.
Poly(A) RNA Selection Kit 100 rxn with up to 5 µg total RNA each or 10 rxn with up to 100 µg each 039.100

Download

User Guide – released 26.11.2015
Product Flyer

Material Safety Datasheets

MSDS information for Poly(A) RNA Selection Kit