Product Description

After extraction total RNA comprises large amounts of ribosomal RNA (rRNA) which frequently is of little interest. Lexogen’s RiboCop rRNA Depletion Kit enables removal of rRNA from total RNA and is suited for Next Generation Sequencing as well as other demanding RNA analysis applications.

Efficient Elimination of rRNA

RiboCop allows efficient removal of cytoplasmic 28S, 18S, 5.8S, 45S, 5S, as well as mitochondrial mt16S, mt12S ribosomal RNA sequences in human, mouse and rat samples. Thus, sequencing depth is preserved for relevant RNA species.

Simple Workflow

The RiboCop protocol takes 2 hours to complete. No enzymatic reactions or mechanical shearing steps are involved, leaving full-length transcripts intact for downstream processing.

Automation

Magnetic beads are used for depletion and purification steps, rendering the protocol completely automation friendly.

Flexible

RiboCop is offered in different kit sizes as a stand-alone kit (Cat. No. 037.24, 037.96) as well as in combination with SENSE Total RNA-Seq Libray Prep Kit(Cat. No. 042.08, 042.24, 042.96).

Low Input Amount

RiboCop was characterized down to input levels of 10 ng total RNA.

Workflow

Step 1 : Denaturation of total RNA

Affinity probes and total RNA are mixed and denatured.

Step 2 : Hybridization of affinity probes to rRNA

Denatured total RNA and probes are hybridized.

Step 3 : Removal of bound rRNA molecules

Depletion beads are conditioned and used to remove probes along with hybridized ribosomal RNA from solution.

Step 4 : Purification of depleted RNA

The depleted RNA is purified for downstream processing.

FAQs

Ordering Information

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User Guide – update 29.03.2015 (Input amounts extended to 10 ng – 1 µg total RNA)
Application Note

Material Safety Datasheets

MSDS information for RiboCop rRNA depletion kit