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Picoplex-WGA-kit

PicoPLEX WGA kit

Product Code:

The PicoPLEX®WGA kit with patented technology* is designed and optimized for amplification of single copy genomic DNA starting with a single cell.


Product Description

The ability to amplify DNA to yield a highly reproducible library from a single cell is now possible! The PicoPLEX® WGA kit with patented technology* is designed and optimized for amplification of single copy genomic DNA starting with a single cell. The easy-to-use single tube protocol reduces handling errors, dramatically improves time to results and reduces background. PicoPLEX WGA Kit can be also be used with isolated gDNA amounts ranging from less than 6 pg to 50 pg. Accepted for use in array CGH analysis as well as PCR.

Advantages

  • Reproducible results from single cells (or
  • Superior reproducibility of allele representation
  • Easy to use and automate
  • Unambiguous results at all resolutions, microarray, and PCR

Specification

Store at –20 °C.
Guaranteed for 12 months at -20°C in a constant temperature freezer.

Kit selection guide

Platform Applications Sample Input Amount Technology
ThruPLEX® DNA-seq Kit Illumina NGS WGS, WES, Targeted Sequencing, ChIP-seq, RNA-seq FFPE DNA, gDNA, cDNA, Cell-free DNA, ChIP DNA, amplicons 50 pg – 50 ng High sensitivity ligation-based amplification
ThruPLEX® Plasma-seq Kit* Illumina NGS WGS, WES, Targeted Sequencing, ctDNA Analysis Cell-free DNA isolated from plasma <1 ng – 30 ng High sensitivity ligation-based amplification
PicoPLEX® DNA-seq Kit Illumina NGS CNV, Aneuploidy Eukaryotic Cell(s) or gDNA <6 pg – 60pg Quasi-random primed linear amplification
PicoPLEX® WGA Kit Arrays, PCR CNV, Aneuploidy Eukaryotic Cell(s) or gDNA <6 pg – 60 pg DNA Quasi-random primed linear amplification
TransPLEX® C-WTA Kit Arrays, PCR Gene Expression Analysis RNA 10 – 300 ng RNA Quasi-random primed amplification

Performance

Amplification of Single Cells is Reproducible

Amp-of-single-cells-slider-072815
Flow-sorted cancer cells were amplified with the PicoPLEX WGA Kit. Each of the cells (blue lines) amplified at the same rate, and resulted in a similar, predictable yield. The sample containing 0 cells (green lines; no template control) shows very low background.

Reproducible CNV Data

CNV-data-sider-072815-2
Single-blastomere biopsies were amplified using PicoPLEX WGA Kit, labeled and hybridized to BlueGnome’s 24Sure arrays at Genesis Genetics Institute. Note clear indication of CNVs. In 2011, ESHRE clinical trials confirmed accuracy of karyotyping using PicoPLEX technology.

PicoPLEX WGA Kit Outperforms the Competition in Locus-specific q-PCR Assays

Locus-chart-slider-072815-3
Locus-specific q-PCR was used to quantify 48 loci in independent single-cell libraries. Data shown compares results of DNA from two individual samples amplified with (left to right) PicoPLEX WGA Kit, GenomePLEX® and GenomiPhi™ using 10 pg of DNA. More than 90% of the product from PicoPLEX produce identifiable human sequences and this was highly reproduced,

PicoPLEX WGA Kit Single-Tube Workflow

PicoPLEX WGA Kit Technology

Cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the Illumina barcodes.

FAQ

1. What are the recommended DNA inputs for the PicoPLEX WGA Kit?
2.What applications are recommended for PicoPLEX® WGA Kit?
3. Is there a non-stick wash buffer that can be used for single cell wash prior to using PicoPLEX WGA Kit?
4. Are there special requirements for flow sorting?
5. What is the sample input volume for PicoPLEX WGA Kit?
6. Can the product of PicoPLEX WGA Kit be taken straight to NGS?
7.Does Rubicon Genomics have an NGS-ready kit for single cell applications?
8. Sigma-Aldrich sells a GenomePlex WGA kit, is it the same technology as PicoPLEX WGA?
9.What is the expected genomic locus representation and Loss of Heterozygosity (LOH) for PicoPLEX WGA products?
10. Is the product of this technology single- or double-stranded?

Ordering Information

CAT. NO. R30050 PicoPLEX® WGA Kit, 50 reactions
For larger volumes or custom applications, contact info@moldiag.in

Download

PicoPLEX WGA Kit product sheet

PicoPLEX WGA Kit manual

SDS PicoPLEX WGA Kit